Comparing the antimicrobial effects of silver and copper nanoparticles against pathogenic and resistant bacteria of Klebsiella pneumonia, Pseudomonas aeruginosa and Staphylococcus aureus

Yasaman Sadat NABIPOUR, Arman ROSTAMZAD
2.032 1.319

Abstract


Abstract. Topics and Objectives: In this study, the effectiveness of antimicrobial activity of Ag and Cu nanoparticles against Gram positive bacteria and Gram-negative resistant pathogens and common nosocomial infections were observed.Methods: At first, in order for the effect of different concentrations of nanoparticles on bacteria in macrodilution method of 0.01, 0.1, 0.5, 1, 1.5 % of silver and copper nanoparticles (medium + nanoparticles) were prepared and added to 105 cell/ml of each of the bacteria.Dishes containing the treated cultures (bacteria + nanoparticles) and control media were placed in a shaking incubator. Then the OD of the treatment and control cultures + control were determined.Achievements of the paper: In statistical analysis, it was revealed that about nanoparticles of Ag and Cu, 0.5 percent concentration of bactericidal particles are able to remove almost 100% of Klebsiella pneumoniae bacteria, Pseudomonas aeruginosa, Staphylococcus aureus and 0.1 percent concentration of each of the 3 bacteria was bacteriostatic.


Keywords


nanoparticle, macrodilution, bacteriostatic, bactericidal

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