Anti-nociceptive and antiinflammatory effects of the hydroalcoholic extract of the Spergularia Marina (L). Griseb in male mice

Abdolhossein MİRİ, Elahe SHAHRAKİ, Kaveh TABRİZİAN, Soheil OUDİ
2.060 454

Abstract


Abstract. In this study, the anti-nociceptive properties of hydroalcoholic extract of Spergularia Marina (L). Griseb in male mice were carried out using chemical (writhing and formalin) and thermal (hot plate) models of nociception. Cotton pellet- induced granuloma model was used for anti- inflammatory investigations in male mice. Spergularia Marina hydroalcoholic extract (12.5, 25 and 50 mg/kg, oral gavage) reduced time and number of paw licks during the second phase of the formalin test and the number of writhings. This extract did not show any significant analgesia on acute pain in first phase of the formalin test. Also, the effect of the extract (25 and 50 mg/kg, p.o.) in sciatic nerve ligation model provides a confirmation of its probable antineuropathic pain properties. Anti-inflammatory effect of this extract (50 mg/kg) was confirmed by a significant decrease in Cotton pellet weight in male mice.


Keywords


Antinociception, Spergularia Marina, Sciatic Nerve ligation, Formalin test, Inflammation

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References


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Figure 1. Effects of Spergularia marina, morphine and diclofenac in the early (A and B) and late phase ( C and D) of

the formalin test in mice by assessing the licking time and the number of lickings. Each value represents the mean ±

S.E.M. for 7 mice. *p<0.05, **p< 0.01 and ***p<0.001 significantly different from the control animals.

Figure 2. Effects of treatment with Spergularia marina on acetic acid- induced abdominal constrictions in mice. Each

value represents the mean ± S.E.M. for 7 mice. *p<0.05, **p< 0.01 and ***p<0.001 significantly different from the

control animals. Sham nerve ligated

14 days after surgery

Figure 3. Changes in reaction time in sham-operated and nerve ligated mice, 14 days after the sciatic nerve ligation.

Each value represents the mean ± S.E.M. for 7 mice. ***p<0.001 significantly different from the control (sham) - treated animals.

Figures 4. A-E. Latency response of the Spergularia marina treated animals in comparison with the control and

imipramine- treated animals (n = 7, mean ± S.E.M.).

Figs A, B, C, D and E represent assessment of anti-nociception at 0, 30, 60, 90 and 120 min, respectively. *p<0.05,

**p< 0.01 and ***p<0.001 significantly different from the control animals. #p<0.05 significantly different from the

imipramine- treated animals.

Figure 5. Anti-inflammatory activity of Spergularia marina in cotton pellet- induced granuloma formation (n = 7,

mean ± S.E.M.). *p<0.05 and **p< 0.01significantly different from the control animals.