Effects of acetlysalicylic acid with indole-3-acetic acid on rooting and pigmentation in Amygdalus L.

Gülçin Beker Akbulut, Emel Yiğit
2.544 1.707


Vegetative propagation is a key step, playing an important role in the succesful production of elite clones. The use of plant hormanes can increase the rroting capacity of cuttings. In this experiment, we investigated whether exogenously applied acetylsalicylic acid (ASA) with indole-3-acetic acit (IAA) (50, 100 mg/L) through the rooting medium could increase effects on Amygdalus spp or not. In the experiment, one year old semihardwood shoot
cuttings were used. The highest callus formation was determined on the applied cuttings with 50 mg/L ASA with IAA in all groups. Rooting among the application groups in A. lycioides was observed around 10% in 50 mg/L ASA
with IAA treatment group. Pigmentation contents in cuttings were found to be important in a time-dependent fashion.


Rooting, acetylsalicylic acid, indole-3-acetic acid,callus

Full Text:

PDF (Türkçe)


Lingdi, L. and Bartholomew, B.,48. Amygdalus Linnaeus, Sp. Pl. 1: 472. 1753, Flora of China, 9 (2003) 391-395.

Edstromand, J.P. and Viveros, M., Almond production manual. In: division of agricultural and natural resources, Micke, W.C. (ed.). Univ. of California Publication, (1996) 3364.

Dokuzoguz, M. ve Gulcan, R., Almond growth and obstacles, Tubitac Publications (1979) 80.

Ercisli, S., A short review of the fruit germplasm resources of Turkey, Genetic Resources and Crop Evolution, 51 (2004) 419-435.

Perez-Tornero, O., Egea, J., Vanoostende, A. and Burgos L., Assessment of factors affecting adventitious shoot regeneration from in vitro cultured leaves of apricot, Plant Science, 158 (2000) 61-70.

Hammatt, N. and Grant N.J., Micropropagation of mature British wild cherry, Plant Cell Tissue and Organ Culture, 47 (1997) 103-110.

Hammerschlag, F.A., Bauchan, G.R. and Scorza, R., Factors influencing in vitro multiplication and rooting of peach cultivars, Plant Cell Tissue and Organ Culture, 8 (1987) 235-2

Aier, N.B., Sharma, S.D., Micropropagation in some plum cultivars, Fruit Science Reports, 17 (1990) 57-63.

Kester, D.E and Gradziel, T.M. and Grassely C., Almonds.Acta Horticulturae, 290 (1990) 699-75

Caboni, E. and Damiano, C., Rooting in two Almond genotypes, Plant Science, 96 (1994) 163-16

Caboni, E., Tonelli, M.G., Lauri, P., Lacovacci, P., Kevers, C., Damiano, C. and Gaspar T., Biochemical aspects of Almond microcuttings related to in vitro rooting ability, Biologia Plantarum, 39 (1997) 91-97.

Ainsley, P.J., Collins, G.G. and Sedgley M., In vitro rooting of Almond (Prunus dulcis Mill.), In vitro Cellular & Developmental Biology-Plant, 37 (2001) 778-785.

Nicotra, A. and Pellegrini, M., Almond rootstock breeding for easy propagation. Options méditerrannées, Série Séminaires, 5 (1989) 51-60.

Steven, T.L., Alan, G.S. and Wesley, P.H., Differential gene expression in response to auxin treatment in the wild type and Rac, an adventitious rooting-incompetent mutant of tobacco, Plant Physiology, 114 (1997) 1197-1206.

Pijut, P.M. and Espinosa, A.C., Development of a rooted cutting propagation method for Prunus serotina, The International Plant Propagators` Society Combined Proceedings, 54 (2005) 129-131.

Hartmann, H.T. and Kester, D.E., Plant Propagation: Principles and Practices. Prentice-Hall, Englewood Cliffs, New Jersey 1975.

Kling, G.J. and Meyer, M.M., Effect of phenolic compounds and indole acetic acid and adventitious root initiation in Phaseouls aureus, Acer saccharinum and Acer griseum, Horticultural Science, 18 (1983) 352-354.

Kang, S.M., Jung, H.Y., Kang, Y.M., Dae-Jin, Y., Bahk, J.D., Yang, J.K. and Choi, M.S., Effects of methyljasmonate and salicylic acid on the production of tropane alkaloids and the expression of PMT and H6H in adventitious root cultures of Scopolia parviflora, Plant Science, 166 (2004) 745-751.

Arfan, M., Athar, H.R. and Ashraf, M., Does exogenous application of salicylic acid through the rooting medium modulate growth and photosynthetic capacity in two differently adapted spring wheat cultivars under salt stress, Journal of Plant Physiology, 164 (2007) 68569

Leslie, C.A. and Romani, R.J. Inhibition of ethylene biosynthesis by salicylic acid, Plant Physiology, 88 (1988) 833-837.

Mandal, S., Nirupama, M. and Adinpunya, M., Salicylic acid-induced resistance to Fusarium oxysporum f. sp. Lycopersici in tomato, Plant Physiology and Biochemistry, 47 (2009) 642-649.

Khodary, S.E.A., Effect of salicylic acid on growth, photosynthesis and carbohydrate metabolism in salt stressed maize plants,International Journal of Agriculture and Biology, 6 (2004) 5-8.

Koch, J.R., Creelman, R.A., Eshita, S.M., Seskar, M., Mullet, J.E. and Davis, K.R., Ozone sensitivity in hybrid poplar correlates with insensitivity to both salicylic acid and Jasmonic Acid. The Role of Programmed Cell Death in Lesion Formation, Plant Physiology, 123 (2000)4 87-49

El-Tayeb, M.A., Response of barley grains to the interactive effect of salinity and salicylic acid,Plant Growth Regulation, 45 (2005) 215-224.

Raskin, I., Skubatz, H., Tang, W. and Meeuse, B.J.D.,Salicylic acid levels in thermogenic and non-thermogenic plants, Annals of Botany, 66 (1990) 369-373.

Senaratna, T., Touchell, D., Bunn, E. and Dixon K., Acetylsalicylic acid (Aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant GrowthRegulation, 30 (2000) 157-161.

Raskin I., Role of salicylic acid in plants,Annual Review of Plant Physiology and Plant Molecular Biology, 43 (1992) 439-463.

Lopez-Delgado, H., Dat, J.F, Foyer, C.H. and Scott, I.M., Induction of thermotolerance in potato microplants by acetylsalicylic acid and H 2 O 2, Journal of Experimental Botany, Vol. April 49:321 (1998) 713-720.

Hartmann, H.T., Kester, D.E., Davies, F.T. and Geneve R.L., Plant Propagation Principles and Practices, 5th ed. Prentice-Hall, New Jersey. 2001.

Kelen, M. and Ozkan G., Relationships between rooting ability and changes of endogenous IAA and ABA during the rooting of hardwood cuttings of some grapevine rootstocks, Eur. J. Hort. Sci., 68 (2003) 8-13.

Negash, L., Vegetative propagation of the threatened African wild olive [Olea europaea L.subsp. cuspidata (Wall. ex DC.) Ciffieri]. New Forest, 26 (2003) 137-146.

Wiesman, Z. and Lavee, S., Enhancement of stimulatory effects on rooting of olive cultivar stem cuttings, Sci Hort, 62 (1995a) 189-198.

Denaxa, N.K., Vemmos, S.N. and Roussos, P.A., The role of endogenous carbohydrates and seasonal variation in rooting ability of cuttings of an easy and a hard to root olive cultivars (Olea europaea L.), Scientia Horticulturae 143 (2012) 9-28.

Hoagland, D.R. and Arnon, D.I. The water culture method for growing plants without soil, California Agr Expt Sta Circ, 37 (1938).

De-Kok, L. and Graham M., Levels of pigments, soluble proteins, amino acids and sulfhydryl compounds in foliar tissue of Arabidopsis thaliana during dark induced and natural senesence,Plant Physiology and Biochemistry, 27 (1989) 203-209.

Lichtenthaler K. and Welburn A.R., Determination of total carotenoids andchlorophylls a and b of leaf extracts in different solvents, Botanisches Institut der Univeristat, Kaiserstran βe 12, Postfach, (1983).

Duncan, D.B., Multiple Range and Multiple F tests. Biometrics, 11:1 (1955).

Mneney, E.E. and Mantell, S.E. Effect of deliberate wounding and natural cashew gum extracts in improving rooting and resilience of cashew cuttings post-excision, Research & Training Newsletter, Dar es Salam, 18 (2003) 8-11.

Martin, K.P. Plant regeneration through direct somatic embryogenesis on seed coat explants of Cashew (Anacardium occidentale L.), Scientia Horticulturae, Wageningen, 98 (2003) 299-304.

Lopes,R.L, Cavalcante, I.H.L, Oliveira, I.V.D.M.,and Martins A.B.G., Indol butyric acid levels on cashew cloning by air-layering process,Revista Brasileira de Fruticultura, JaboticabalSP, 27 (2005) 517-518.

Antonopoulou, C., Dimassi, K., Therios, I., Chatzissavvidis, C. and Tsirakoglou, V., Inhibitory effect of riboflavin (Vitamin B 2 ) on the in vitro rooting and nutrient concentration of explants of peach rootstock GF 677 (Prunus amygdalus x P. persica), Scientia Horticulturae, 106 (2005) 268-272.

Nandi, S.K., Rikhari, H.C., Nadeem, M. and Palni, L.M.S., Physiology and Molecular Biology of Plants, 3 (1997) 15.

Singh, S.P., Effect of non-auxinic chemicals on root formation in some ornamental plant cuttings, Advances in Horticultural Science, 3 (1993) 207-210.

Li, L. and Li, L., Effects of recorcinol and salicylic acid on the formation of adventitious Roots on hypocotyl cutting of Vigna radiata,Journal of Topical an Subtropical Botany, 3 (1995) 67-7

Isfendiyaroglu, M. and Ozeker, E., Rooting of Olea europaea ‘Domat’ cuttings by auxin and salicylic acid treatments, Pakistan Journal of Botany, 40 (2008) 1135-1141.

Bojarczuk, T. and Jankiewicz, S.L., Influence of phenolic substances on rooting of softwood cuttings of Populus alba L., P. canescens Sm., Acta Agrobotanica, 28 (1975) 1211 10