The effects of allograft combined with ozone therapy on regeneration of calvarial defects in rats

Hülya TOKER, Hakan ÖZDEMİR, Turan Emre KUZU, Hatice ÖZER
1.058 149


Aim: Ozone accelerates wound healing and increases the blood supply to that in previous studies reported in the literature.  In this study, we claim that  ozone therapy   can increase the bone  regeneration of the combined used  with  bone  allograft calvarial defects in rats.

Material-Method: 12 male Wistar Rats were used in this study.  Critical bone defects in the rat skulls were created by means of  trephan bur(Mıs Implant Tech, Shlomi, Israel), opening a 5 mm diameter defect on the right side of the naso-frontal region of the skull. The rats were separated into 4 groups in random order;1-Control defect (n=6),2-Ozone application into the control defect (control+ ozone) group (n=6),3-Allograft group (n=6) 4-Ozone Combined with Allograft application (allograft + ozone) (n=6). The defects  were administered  at  80% density ozone for 14  days (Ozonytron, W&H, Bürmoos, Austria). Rats sacrifice at 8. week

Results: The osteoblast numbers were not significantly different in the control and control + ozone groups in the 8th week .Allograft + ozone group, the osteoblast numbers  increased at a statistically significant  in the 8th week when compared with the allograft group. Bone area measurements were statistically analyzed among the groups in the 8th week, it was observed that the allograft + ozone group showed a significant increase in the bone area amount  while no statistical significance was observed in the ozone-treated contol  defects, and new bone formation was observed less in the control defects.

Conclusions: The results of this study , allograft + ozone combination increases the osteoblast number and new bone area at an important level when compared with the allograft group alone.


Keywords:Bone healing,autogenous grafts, allografts, ozone therapy,calvarial defects

Full Text:



Jensen, S.S., et al., Bone healing and graft resorption of autograft, anorganic bovine bone and beta-tricalcium phosphate. A histologic and histomorphometric study in the mandibles of minipigs. Clin Oral Implants Res, 2006. 17(3): p. 237-43.

Oh, K.C., et al., The influence of perforating the autogenous block bone and the recipient bed in dogs. Part I: a radiographic analysis. Clin Oral Implants Res, 2011. 22(11): p. 1298-302.

Reynolds, M.A. and G.M. Bowers, Fate of demineralized freeze-dried bone allografts in human intrabony defects. J Periodontol, 1996. 67(2): p. 150-7.

Salyer, K.E., et al., Cranioplasty in the growing canine skull using demineralized perforated bone. Plast Reconstr Surg, 1995. 96(4): p. 770-9.

Paper, A.A.P.P., Tissue Banking Of Bone Allografts Used in Periodontal Regeneration. j.periodontol, 2001. 72: p. 834-8.

Shigeyama, Y., et al., Commercially-prepared allograft material has biological activity in vitro. J Periodontol, 1995. 66(6): p. 478-87.

Messora, M.R., et al., Effect of platelet-rich plasma on the healing of mandibular defects treated with fresh frozen bone allograft: a radiographic study in dogs. J Oral Implantol, 2014. 40(5): p. 533-41.

The potential role of growth and differentiation factors in periodontal regeneration. J Periodontol, 1996. 67(5): p. 545-53.

Fideler BM, V.C.J., Moore T, Li Z, Rasheed S, Effects of gamma irradiation on the human immunodeficiency virus. A study in frozen human bone-patellar ligament-bone grafts obtained from infected cadavera. J Bone Joint Surg Am, 1994. 76-A(7): p. 1032-5.

Cornu, O., et al., Freeze-dried irradiated bone brittleness improves compactness in an impaction bone grafting model. Acta Orthop Scand, 2004. 75(3): p. 309-14.

Garrett, S., Periodontal regeneration around natural teeth. Ann Periodontol, 1996. 1(1): p. 621-66.

Babbush, C.A., The use of a new allograft material for osseous reconstruction associated with dental implants. Implant Dent, 1998. 7(3): p. 205-12.

Babbush, C.A., Histologic evaluation of human biopsies after dental augmentation with a demineralized bone matrix putty. Implant Dent, 2003. 12(4): p. 325-32.

Guerra OC, C.S., Jordan MEM, Vazquez and TC., Aplicación de la ozonoterapia en etratamiento de la alveolitis. Revista Cubana de Estomatologia, 1995. 34: p. 21-4.

Petrucci, M.T., et al., Role of ozone therapy in the treatment of osteonecrosis of the jaws in multiple myeloma patients. Haematologica, 2007. 92(9): p. 1289-90.

Ozdemir, H., et al., Effect of ozone therapy on autogenous bone graft healing in calvarial defects: a histologic and histometric study in rats. J Periodontal Res, 2013. 48(6): p. 722-6.

Frame, J.W., A convenient animal model for testing bone substitute materials. J Oral Surg, 1980. 38(3): p. 176-80.

Bays, R.A., The influence of systemic bone disease on bone resorption following mandibular augmentation. Oral Surg Oral Med Oral Pathol, 1983. 55(3): p. 223-31.

Higuchi, T., et al., Bone regeneration by recombinant human bone morphogenetic protein-2 in rat mandibular defects. An experimental model of defect filling. J Periodontol, 1999. 70(9): p. 1026-31.

Dupoirieux, L., et al., Comparative study of three different membranes for guided bone regeneration of rat cranial defects. Int J Oral Maxillofac Surg, 2001. 30(1): p. 58-62.

Ivanovski, S., et al., Transcriptional profiling of "guided bone regeneration" in a critical-size calvarial defect. Clin Oral Implants Res, 2011. 22(4): p. 382-9.

Kochi, G., et al., Analysis on the guided bone augmentation in the rat calvarium using a microfocus computerized tomography analysis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2009. 107(6): p. e42-8.

Notodihardjo, F.Z., et al., Bone regeneration with BMP-2 and hydroxyapatite in critical-size calvarial defects in rats. J Craniomaxillofac Surg, 2012. 40(3): p. 287-91.

Ellegaard, B., Bone grafts in periodontal attachment procedures. J Clin Periodontol, 1976. 3(5): p. 1-54.

Mellonig, J.T., et al., Clinical evaluation of freeze-dried bone allografts in periodontal osseous defects. J Periodontol, 1976. 47(3): p. 125-31.

Galgut, P.N., et al., A 4-year controlled clinical study into the use of a ceramic hydroxylapatite implant material for the treatment of periodontal bone defects. J Clin Periodontol, 1992. 19(8): p. 570-7.

Dragoo, M.R. and H.C. Sullivan, A clinical and histological evaluation of autogenous iliac bone grafts in humans. I. Wound healing 2 to 8 months. J Periodontol, 1973. 44(10): p. 599-613.

Reynolds, M.A., et al., The efficacy of bone replacement grafts in the treatment of periodontal osseous defects. A systematic review. Ann Periodontol, 2003. 8(1): p. 227-65.

Schmitt, C.M., et al., Histological results after maxillary sinus augmentation with Straumann(R) BoneCeramic, Bio-Oss(R), Puros(R), and autologous bone. A randomized controlled clinical trial. Clin Oral Implants Res, 2013. 24(5): p. 576-85.7

Froum, S.J., et al., Comparison of mineralized cancellous bone allograft (Puros) and anorganic bovine bone matrix (Bio-Oss) for sinus augmentation: histomorphometry at 26 to 32 weeks after grafting. Int J Periodontics Restorative Dent, 2006. 26(6): p. 543-51.

Noumbissi, S.S., et al., Clinical, histologic, and histomorphometric evaluation of mineralized solvent-dehydrated bone allograf (Puros) in human maxillary sinus grafts. J Oral Implantol, 2005. 31(4): p. 171-9.

Mollica P, H.R. Integrating oxygen/ ozone therapy into your practice. 2010 Available from: http://www. Toxin free smile. Dom/ images/ozone integrating % 20 oxygen ozone

Journal of periodontology Suppl.3: p. 117-135.

Kang, Y.H., et al., Platelet-rich fibrin is a Bioscaffold and reservoir of growth factors for tissue regeneration. Tissue Eng Part A, 2011. 17(3-4): p. 349-59.

Kazancioglu, H.O., S. Ezirganli, and M.S. Aydin, Effects of laser and ozone therapies on bone healing in the calvarial defects. J Craniofac Surg, 2013. 24(6): p. 2141-6.

Kazancioglu, H.O., E. Kurklu, and S. Ezirganli, Effects of ozone therapy on pain, swelling, and trismus following third molar surgery. Int J Oral Maxillofac Surg, 2014. 43(5): p. 644-8.

Stubinger, S., R. Sader, and A. Filippi, The use of ozone in dentistry and maxillofacial surgery: a review. Quintessence Int, 2006(37(5)): p. p. 353-9.

Bocci, V., et al., Studies on the biological effects of ozone: 7. Generation of reactive oxygen species (ROS) after exposure of human blood to ozone. J Biol Regul Homeost Agents, 1998. 12(3): p. 67-75.

This work is licensed under a Creative Commons Attribution-Gayriticari-NoDerivs 3.0 Unported Lisansı