The effects of allograft combined with ozone therapy on regeneration of calvarial defects in rats

Hülya TOKER, Hakan ÖZDEMİR, Turan Emre KUZU, Hatice ÖZER
2.110 299


Aim: Ozone accelerates wound healing and increases the blood supply to that in previous studies reported in the literature.  In this study, we claim that  ozone therapy   can increase the bone  regeneration of the combined used  with  bone  allograft calvarial defects in rats.

Material-Method: 12 male Wistar Rats were used in this study.  Critical bone defects in the rat skulls were created by means of  trephan bur(Mıs Implant Tech, Shlomi, Israel), opening a 5 mm diameter defect on the right side of the naso-frontal region of the skull. The rats were separated into 4 groups in random order;1-Control defect (n=6),2-Ozone application into the control defect (control+ ozone) group (n=6),3-Allograft group (n=6) 4-Ozone Combined with Allograft application (allograft + ozone) (n=6). The defects  were administered  at  80% density ozone for 14  days (Ozonytron, W&H, Bürmoos, Austria). Rats sacrifice at 8. week

Results: The osteoblast numbers were not significantly different in the control and control + ozone groups in the 8th week .Allograft + ozone group, the osteoblast numbers  increased at a statistically significant  in the 8th week when compared with the allograft group. Bone area measurements were statistically analyzed among the groups in the 8th week, it was observed that the allograft + ozone group showed a significant increase in the bone area amount  while no statistical significance was observed in the ozone-treated contol  defects, and new bone formation was observed less in the control defects.

Conclusions: The results of this study , allograft + ozone combination increases the osteoblast number and new bone area at an important level when compared with the allograft group alone.


Keywords:Bone healing,autogenous grafts, allografts, ozone therapy,calvarial defects

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