Changes emerging with calcineurin inhibition in Caenorhabditis

Mustafa Tosun, Sibel Berksoy Hayta, Rukiye Güner, Melih Akyol, Sedat Özçelik
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Abstract


Background: It is known that calcineurin inhibition prolongs life in individuals with Caenorhabditis elegans (C. elegans). An important part of C. elegans genes shows great similarity to human genes. Aim: This study aims to evaluate the effects of a calcineurin inhibitor on vital functions in C. elegans individuals.

Study Design: Animal experimentation

 Methods:  In this study, solutions of cyclosporine A which is a calcineurin inhibitör 1 μM (1.dose), 0.1 μM (2.dose), 0.01 μM (3.dose) and 0.001 μM (4.dose) were prepared and 1 ml from each solution was taken and added to petri dishes, containing 10 ml Nematode Growth Media (NGM). The control group was fed the medium which was not added cyclosporine. The indicated doses of cyclosporine whose synchronization has been completed were transferred to petri dishes in order that each would have 20 pieces. C. elegans were observed using bottom light stereo microscop that provided 6X-50X magnification. Live animals at the same time every day were counted and compared with the control group until all C. elegans in all petri dishes died.

Results: The results have showed that there was a statistically significant increase in life span periods of C. elegans exposed to cyclosporin depending on dose increase (p<0,05).  It has been found out that physical development of C. elegans exposed to increased doses of cyclosporin decreased significantly (p<0,05). The increase in doses of cyclosporin have not caused statitically significant change in the number of pharynx pumping (p>0,05).

Conclusion: Calcineurin inhibition slows down the developmental process in C. elegans and prolongs survival, and may produce positive effects on aging.


Keywords


: Caenorhabditis elegans, calcineurin, cyclosporine A, skin aging, skin aging model

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