Effect of the cyclooxygenase-2 inhibitor tenoxicam on pentylenetetrazole-induced epileptic seizures in rats
METHODS: Eighteen Wistar Albino male rats (220±20 g) were divided into three groups: control (n=6), 10 mg/kg/day tenoxicam (n=6) and, 20 mg/kg/day tenoxicam (n=6). Tenoxicam was administered intramuscularly for ten days. On the tenth day, pentylenetetrazol (PTZ) was injected intraperitoneally at 70 mg/kg after 45 minutes of drug administration and the animals were observed for 30 min. Stages were determined according to the Racine seizure scale (RC) and the first myoclonic jerk time (FMJ) was recorded in seconds. After completing procedure, whole brain tissues were removed and stained with toluidine blue stain. The number of dark neurons with chromatin aggregation in hypocampal CA1 and dentate gyrus (DG) was determined as percentage.
RESULTS: Epileptic behavior were evaluated according to the RC, 10 mg/kg of tenoxicam significantly reduced the seizure stage compared to the control (p<0,05). In addition, 10 mg/kg tenoxicam significantly increased the FMJ compared to the control (p<0,05). According to the histopathological findings, neuronal damage was increased in CA1 region of 20 mg/kg of tenoxicam group compared to control, whereas neuronal damage was reduced significantly in the dentate gyrus of 10 mg/kg and 20 mg/kg of tenoxicam groups (p<0,05).
CONCLUSIONS: This study shows that dose-dependent administration of tenoxicam might have a potential to reduce epileptic seizures and post-seizure neuron damage.
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