A study on the antioxidant, antimicrobial and cytotoxic activity of Thymbra spicata L. var. spicata ethanol extract
Aim. Thymbra species are widely used in Turkish folk medicine as salad or herbal tea for antioxidant, antiseptic, antitussive, carminative, anti-inflammatory and antimicrobial activities. The present study was aimed to determine antioxidant, antimicrobial and cytotoxic properties of ethanol crude extract of Thymbra spicata var. spicata. Leaves.
Methods. The antioxidant properties of ethanol extracts of Thymbra spicata were investigated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis[3-ethylbenzthiazoline]-6-sulfonic acid (ABTS) radical scavenging activity, total flavonoid and total phenolic content analysis. Antimicrobial activity of the plant extracts were determined using the microdilution method, while proliferative activity were evaluated by MTT assay on MCF-7 breast cancer and PC3 porstat cancer cell line.
Results. Total phenol and total flavonoid content were 216.75 ± 0.017 (equivalent of Gallic acid mg/g dry extract) and 78.17 ± 0.063 (equivalent of Quercetin mg/g), IC50 value for DPPH and ABTS scavenging activity was 16.91 µg/mL and 5.2 ± 0.009 µg/mL, respectively. Antimicrobial activity observed for the extract ranging between 2.5 and 5mg/ml as MIC value against tested microorganism. MTT assay was employed for evaluation of cytotoxic activity, ethanol extract demonstrated growth inhibitory effect against MCF-7 cancer cells with IC50 value 340 µg/mL.
Conclusion. According to the obtained results, it can be said that the antioxidant and antimicrobial activities, together with the ability of cytotoxicity, provide some support for the T.spicata’s traditional use.
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